Quantitative changes in protein expression of cadmium-exposed poplar plants.
Identifieur interne : 003835 ( Main/Exploration ); précédent : 003834; suivant : 003836Quantitative changes in protein expression of cadmium-exposed poplar plants.
Auteurs : Pol Kieffer [Luxembourg (pays)] ; Jacques Dommes ; Lucien Hoffmann ; Jean-François Hausman ; Jenny RenautSource :
- Proteomics [ 1615-9861 ] ; 2008.
Descripteurs français
- KwdFr :
- Cadmium (métabolisme), Cadmium (pharmacologie), Carbone (métabolisme), Culture hydroponique (MeSH), Distribution tissulaire (MeSH), Facteurs temps (MeSH), Feuilles de plante (composition chimique), Feuilles de plante (croissance et développement), Feuilles de plante (effets des médicaments et des substances chimiques), Feuilles de plante (métabolisme), Populus (anatomie et histologie), Populus (croissance et développement), Populus (effets des médicaments et des substances chimiques), Populus (métabolisme), Protéines (génétique), Protéines (métabolisme), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Spectrométrie de masse MALDI (MeSH), Stress oxydatif (effets des médicaments et des substances chimiques), Tiges de plante (composition chimique), Tiges de plante (croissance et développement), Tiges de plante (effets des médicaments et des substances chimiques), Tiges de plante (métabolisme).
- MESH :
- anatomie et histologie : Populus.
- composition chimique : Feuilles de plante, Tiges de plante.
- croissance et développement : Feuilles de plante, Populus, Tiges de plante.
- effets des médicaments et des substances chimiques : Feuilles de plante, Populus, Régulation de l'expression des gènes, Stress oxydatif, Tiges de plante.
- génétique : Protéines.
- métabolisme : Cadmium, Carbone, Feuilles de plante, Populus, Protéines, Tiges de plante.
- pharmacologie : Cadmium.
- Culture hydroponique, Distribution tissulaire, Facteurs temps, Spectrométrie de masse MALDI.
English descriptors
- KwdEn :
- Cadmium (metabolism), Cadmium (pharmacology), Carbon (metabolism), Gene Expression Regulation (drug effects), Hydroponics (MeSH), Oxidative Stress (drug effects), Plant Leaves (chemistry), Plant Leaves (drug effects), Plant Leaves (growth & development), Plant Leaves (metabolism), Plant Stems (chemistry), Plant Stems (drug effects), Plant Stems (growth & development), Plant Stems (metabolism), Populus (anatomy & histology), Populus (drug effects), Populus (growth & development), Populus (metabolism), Proteins (genetics), Proteins (metabolism), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Time Factors (MeSH), Tissue Distribution (MeSH).
- MESH :
- chemical , genetics : Proteins.
- chemical , metabolism : Cadmium, Carbon, Proteins.
- chemical , pharmacology : Cadmium.
- anatomy & histology : Populus.
- chemistry : Plant Leaves, Plant Stems.
- drug effects : Gene Expression Regulation, Oxidative Stress, Plant Leaves, Plant Stems, Populus.
- growth & development : Plant Leaves, Plant Stems, Populus.
- metabolism : Plant Leaves, Plant Stems, Populus.
- Hydroponics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Time Factors, Tissue Distribution.
Abstract
Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification. Additionally, growth and other physiological parameters were monitored during the experiment. Treated plants exhibited an inhibition of growth and visual symptoms appeared after 7 days. A significant accumulation of Cd in all organs was recorded by ICP-MS analysis. A number of changes in the expression of proteins with various functions were identified; in particular a decreased abundance of oxidative stress regulating proteins, whereas pathogenesis-related proteins showed a drastic increase in abundance. Furthermore, a large number of proteins involved in carbon metabolism showed a decrease in abundance, while proteins involved in remobilizing carbon from other energy sources were upregulated. In conclusion, the negative effect of Cd could be explained by a deleterious effect on protein expression from the primary carbon metabolism and from the oxidative stress response mechanism. Accumulation of Cd in stems of poplar, coupled with a low impact of Cd on physiological parameters, promotes the use of poplar trees for phytoremediation purposes.
DOI: 10.1002/pmic.200701110
PubMed: 18563750
Affiliations:
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first="Jean-François" last="Hausman">Jean-François Hausman</name></author><author><name sortKey="Renaut, Jenny" sort="Renaut, Jenny" uniqKey="Renaut J" first="Jenny" last="Renaut">Jenny Renaut</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium (metabolism)</term><term>Cadmium (pharmacology)</term><term>Carbon (metabolism)</term><term>Gene Expression Regulation (drug effects)</term><term>Hydroponics (MeSH)</term><term>Oxidative Stress (drug effects)</term><term>Plant Leaves (chemistry)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (metabolism)</term><term>Plant Stems (chemistry)</term><term>Plant Stems (drug effects)</term><term>Plant Stems (growth & development)</term><term>Plant 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Desorption-Ionization</term><term>Time Factors</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Culture hydroponique</term><term>Distribution tissulaire</term><term>Facteurs temps</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification. Additionally, growth and other physiological parameters were monitored during the experiment. Treated plants exhibited an inhibition of growth and visual symptoms appeared after 7 days. A significant accumulation of Cd in all organs was recorded by ICP-MS analysis. A number of changes in the expression of proteins with various functions were identified; in particular a decreased abundance of oxidative stress regulating proteins, whereas pathogenesis-related proteins showed a drastic increase in abundance. Furthermore, a large number of proteins involved in carbon metabolism showed a decrease in abundance, while proteins involved in remobilizing carbon from other energy sources were upregulated. In conclusion, the negative effect of Cd could be explained by a deleterious effect on protein expression from the primary carbon metabolism and from the oxidative stress response mechanism. Accumulation of Cd in stems of poplar, coupled with a low impact of Cd on physiological parameters, promotes the use of poplar trees for phytoremediation purposes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18563750</PMID><DateCompleted><Year>2008</Year><Month>08</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>12</Issue><PubDate><Year>2008</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Quantitative changes in protein expression of cadmium-exposed poplar plants.</ArticleTitle><Pagination><MedlinePgn>2514-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.200701110</ELocationID><Abstract><AbstractText>Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification. Additionally, growth and other physiological parameters were monitored during the experiment. Treated plants exhibited an inhibition of growth and visual symptoms appeared after 7 days. A significant accumulation of Cd in all organs was recorded by ICP-MS analysis. A number of changes in the expression of proteins with various functions were identified; in particular a decreased abundance of oxidative stress regulating proteins, whereas pathogenesis-related proteins showed a drastic increase in abundance. Furthermore, a large number of proteins involved in carbon metabolism showed a decrease in abundance, while proteins involved in remobilizing carbon from other energy sources were upregulated. In conclusion, the negative effect of Cd could be explained by a deleterious effect on protein expression from the primary carbon metabolism and from the oxidative stress response mechanism. Accumulation of Cd in stems of poplar, coupled with a low impact of Cd on physiological parameters, promotes the use of poplar trees for phytoremediation purposes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kieffer</LastName><ForeName>Pol</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department Environment and Agrobiotechnologies, Centre de Recherche Public-Gabriel Lippmann, Belvaux, Luxembourg.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dommes</LastName><ForeName>Jacques</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Hoffmann</LastName><ForeName>Lucien</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Hausman</LastName><ForeName>Jean-François</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Renaut</LastName><ForeName>Jenny</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018527" MajorTopicYN="N">Hydroponics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014018" MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>8</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18563750</ArticleId><ArticleId IdType="doi">10.1002/pmic.200701110</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Luxembourg (pays)</li></country></list><tree><noCountry><name sortKey="Dommes, Jacques" sort="Dommes, Jacques" uniqKey="Dommes J" first="Jacques" last="Dommes">Jacques Dommes</name><name sortKey="Hausman, Jean Francois" sort="Hausman, Jean Francois" uniqKey="Hausman J" first="Jean-François" last="Hausman">Jean-François Hausman</name><name sortKey="Hoffmann, Lucien" sort="Hoffmann, Lucien" uniqKey="Hoffmann L" first="Lucien" last="Hoffmann">Lucien Hoffmann</name><name sortKey="Renaut, Jenny" sort="Renaut, Jenny" uniqKey="Renaut J" first="Jenny" last="Renaut">Jenny Renaut</name></noCountry><country name="Luxembourg (pays)"><noRegion><name sortKey="Kieffer, Pol" sort="Kieffer, Pol" uniqKey="Kieffer P" first="Pol" last="Kieffer">Pol Kieffer</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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